A library of reusable designs created and shared by the Genetic Constructor community.
308215 Base Pairs
Templates for the EMMA assembly standard
Bacterial expression plasmids
Library of commonly used plasmids and backbones for basic cloning or bacterial expression
Constructs and part libraries used for our interactive tutorials
Library of plasmids for lentivirus generation
List blocks for mammalian or prokaryotic promoters, fluorescent markers, tags and drug resistance genes.
CRISPRainbow Multiplex Labeling Kit
CRISPRainbow is a system that uses dead Cas9 (dCas9) and gRNA scaffolds that bind fluorescent proteins. This technique was used to simultaneously image six colors in order to label chromsomal loci. To generate the various colors, different hairpins (MS2, PP7, or boxB) were inserted into stem loops of the gRNA. Colors were generated by recruiting pairs of fluorescent proteins (BFP, GFP, or RFP) that were fused to RNA hairpin binding domains. Blue: MS2-MS2 Green: PP7-PP7 Red: boxB-boxB Cyan: MS2-PP7 Yellow: PP7-boxB Magenta: boxB-MS2 White: boxB-MS2-PP7 Learn more: http://www.umassmed.edu/news/news-archives/2016/04/building-a-crispr-rainbow/ Multiplexed labeling of genomic loci with dCas9 and engineered sgRNAs using CRISPRainbow. Ma H, Tu LC, Naseri A, Huisman M, Zhang S, Grunwald D, Pederson T. Nat Biotechnol. 2016 Apr 18. doi: 10.1038/nbt.3526. PubMed PMID: 27088723. Order kit: https://www.addgene.org/kits/pederson-crisprainbow/